mouse monoclonal rv c2  (Developmental Studies Hybridoma Bank)

Journal: iScience

Article Title: Generation of self-organized autonomic ganglion organoids from fibroblasts

doi: 10.1016/j.isci.2023.106241

Figure Lengend Snippet: Double-immunostaining was performed after 10 days of co-culture using antibodies against cTnT and Tuj1.

Article Snippet: Mouse monoclonal anti- Bovine troponin T (cTnT) , DSHB , Cat# ct3, RRID: AB_528495.

Techniques:

Journal: iScience

Article Title: Generation of self-organized autonomic ganglion organoids from fibroblasts

doi: 10.1016/j.isci.2023.106241

Figure Lengend Snippet: Double-immunostaining was performed after 10 days of co-culture using antibodies against cTnT and TH.

Article Snippet: Mouse monoclonal anti- Bovine troponin T (cTnT) , DSHB , Cat# ct3, RRID: AB_528495.

Techniques:

Journal: iScience

Article Title: Generation of self-organized autonomic ganglion organoids from fibroblasts

doi: 10.1016/j.isci.2023.106241

Figure Lengend Snippet: APH-induced iAG neurons innervate co-cultured ventricular myocytes (A) Schematic showing that iAG neurons converted from MEFs were co-cultured with neonatal mouse ventricular myocytes (NMVMs). (B) Representative images showing double-immunostaining of co-cultured cells for Tuj1 and the NMVM marker cTnT after co-culture for 7 to 10 days. Shown above and to the right are the ortho views of z stack images (see for the 3D reconstructed image). Above the XY section is the image of the XZ section, marked with a green border; To the right of the XY section is the image of the YZ section, marked with a red border. The projection of the XZ plane on XY and YZ planes is marked by the red line; the projection of the YZ plane onto the other two planes is marked by the green line. (C) Representative image showing double-immunostaining of co-cultured cells for TH and cTnT after co-culture for 7 days. (D and E) representative intensity traces showing a co-cultured NMVM with increased beating rate after the addition of nicotine (1 μM). (F) Statistical analysis of co-cultured NMVMs with increased beating rate after nicotine treatment. Data are presented as means ± SEM (n = 13). ∗∗p < 0.0001. (G and H) Representative intensity traces showing a co-cultured NMVM with reduced beating rate after the addition of nicotine (1 μM). (I) Statistical analysis of co-cultured NMVMs with decreased beating rate after nicotine treatment. Data are presented as means ± SEM (n = 5). ∗p < 0.05.

Article Snippet: Mouse monoclonal anti- Bovine troponin T (cTnT) , DSHB , Cat# ct3, RRID: AB_528495.

Techniques: Cell Culture, Double Immunostaining, Marker, Co-Culture Assay

Journal: iScience

Article Title: Generation of self-organized autonomic ganglion organoids from fibroblasts

doi: 10.1016/j.isci.2023.106241

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti- Bovine troponin T (cTnT) , DSHB , Cat# ct3, RRID: AB_528495.

Techniques: Diagnostic Assay, Recombinant, Sequencing, Software, Imaging

Journal: STAR Protocols

Article Title: Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

doi: 10.1016/j.xpro.2020.100015

Figure Lengend Snippet: Characterization of hiPSC-Derived Cardiomyocytes (A) Immunofluorescence staining of cardiac markers (cTnT, α-Actinin, MHC, and NKX2.5) shows the cardiomyocytes differentiated from iPSCs at day 30. (B) Flow cytometry using cTnT antibody indicates high purity (95%) of cardiomyocytes in the differentiated cells at day 10 after induction of differentiation. Scale bar, 50 μm.

Article Snippet: cTnT (Troponin T) primary mouse monoclonal antibody , Developmental Studies Hybridoma Bank , CT3.

Techniques: Derivative Assay, Immunofluorescence, Staining, Flow Cytometry

Journal: STAR Protocols

Article Title: Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

doi: 10.1016/j.xpro.2020.100015

Figure Lengend Snippet:

Article Snippet: cTnT (Troponin T) primary mouse monoclonal antibody , Developmental Studies Hybridoma Bank , CT3.

Techniques: Recombinant, Membrane